Research papers and code for "D. Racoceanu":
Accurate detection of mitosis plays a critical role in breast cancer histopathology. Manual detection and counting of mitosis is tedious and subject to considerable inter- and intra-reader variations. Multispectral imaging is a recent medical imaging technology, proven successful in increasing the segmentation accuracy in other fields. This study aims at improving the accuracy of mitosis detection by developing a specific solution using multispectral and multifocal imaging of breast cancer histopathological data. We propose to enable clinical routine-compliant quality of mitosis discrimination from other objects. The proposed framework includes comprehensive analysis of spectral bands and z-stack focus planes, detection of expected mitotic regions (candidates) in selected focus planes and spectral bands, computation of multispectral spatial features for each candidate, selection of multispectral spatial features and a study of different state-of-the-art classification methods for candidates classification as mitotic or non mitotic figures. This framework has been evaluated on MITOS multispectral medical dataset and achieved 60% detection rate and 57% F-Measure. Our results indicate that multispectral spatial features have more information for mitosis classification in comparison with white spectral band features, being therefore a very promising exploration area to improve the quality of the diagnosis assistance in histopathology.

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In this paper, we introduced a novel feature extraction approach, named exclusive autoencoder (XAE), which is a supervised version of autoencoder (AE), able to largely improve the performance of nucleus detection and classification on hematoxylin and eosin (H&E) histopathological images. The proposed XAE can be used in any AE-based algorithm, as long as the data labels are also provided in the feature extraction phase. In the experiments, we evaluated the performance of an approach which is the combination of an XAE and a fully connected neural network (FCN) and compared with some AE-based methods. For a nucleus detection problem (considered as a nucleus/non-nucleus classification problem) on breast cancer H&E images, the F-score of the proposed XAE+FCN approach achieved 96.64% while the state-of-the-art was at 84.49%. For nucleus classification on colorectal cancer H&E images, with the annotations of four categories of epithelial, inflammatory, fibroblast and miscellaneous nuclei. The F-score of the proposed method reached 70.4%. We also proposed a lymphocyte segmentation method. In the step of lymphocyte detection, we have compared with cutting-edge technology and gained improved performance from 90% to 98.67%. We also proposed an algorithm for lymphocyte segmentation based on nucleus detection and classification. The obtained Dice coefficient achieved 88.31% while the cutting-edge approach was at 74%.

* 11 pages, 7 figures, 5 tables, conference paper
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